1、分類號密級單位代碼：10019學號：S05070812飛目差≮武等’學位論文貫葉連翹、元寶草懸浮細胞系建立及其金絲桃素類物質的研究EstablishmentofcellsuspensionandmetabolismofhyperieinsinHypericumperforatumandsampsonii研究生：韭擅指導教師：處盈社教撞合作指導教師：丞歪渣申請學位類別：王堂亟專業(yè)領域名稱：盒品生物撞苤研究方向：蘊醛王程所在學院：盒品型堂皇

2、萱差工程堂瞳2007年6月AbstractHyperiumpeoCoratumLandHypericumsampsoniiHancebelongtotheGuttiferaeItstwomainactiveconsitituents(hypcricinandpseudohyporicin)calleffectivelyinhibitdepressionandvirusInordertoobtamhypericins，thisresear

3、chstudiedthecallusandsuspensioncellsystemofHyperiumperforatumLandHypericumsampsoniiHanceThemainresultsa”asfollows：1ResultsshowedthattheoptimalexplantsforHyperiumperforatumLcallusinductingWasStLmI；undertight，thesuitablecu

4、lturemediaWas1／2NMSmediumsupplementedwith05mgr124一D02ragL“1BAAndleafwasthebestexplantforHyper咖msampsoniiHancecallusforming；1／2NMSmediumsupplementedwith06ragL1NAA0IragL1KTWasthemostsuitableforcallusinducting2SucroseWasthe

5、optiInlllncarbonSOorCA絡forcallusproliferating1rvIPwastheopttmelantioxidantthatcouldpreventbrowningincallusofHypenumPenforatumLsubculture。andVcwasthemosteffectiveantioxidantforcallusofHyper／cwnsampsonffHancoBothoftbegrowt

6、hcirclesofthistwoplants’calluswere24d，andhypericiusproductionWasassumedtorelatetocallmgrowthrate3HypcricimmetaboliteWasassumedtelatetocellsuspensionsgrowthinHyperiumperforatumL砌竺翟”=打冀。悲勰刪“嘣最me刪仉d1唧刪咖嘲～～。rM㈣：∥=籌籌一篙籌4Theef

7、fc塒sofeficitorandprecursor011metabolizingofhyporiciusincellofHyperiumPenforatumLand月玨碥rfc“塒sampsoniiHancewe佗studiedTheresultsshowedthatMethyljasmonatea(MeJA)andPhenylalanine(vbe)coulddramaticallypromotehypericiusThismade

8、hypenciusiIlcnmbycomplexregulationpossibleInawordthecellsuspensioncultureofHyperiumperforatumLandHypericumsampson豇HanceWere咖edFollowingsupplementofMeJAandPbethecontentofhypericinsincreasedsignificanflyThatprovidedagoodsy