1、復旦大學碩士學位論文水稻蘋果酸脫氫酶基因的克隆及其功能研究姓名：姜瑞華申請學位級別：碩士專業(yè)：遺傳學指導教師：楊金水2001.5.21復旦大學碩士畢業(yè)論文CloningoftheRiceCytoplasmicMalicDehydrogenaseGene(RcMDH)AndtheStudyofItsFunctionAbstract：MaIicdehydrogenaseisoneofthekeyenzymesoforganismItplay

2、sanimportantroleinmanyphysiologicalprocessesofthecell，suchastheenergymetabolismofthemitoehondria，theaerobicmetabolismofthemalate—aspartateshuttleandthecitricacidcycleandtheperoxidemetabolismintheplantdiseasesresistanceEv

3、eninthephotosynthesisoftheCaplant，MDHalsodosomecrucialworkSothecloningofit’sgenewillbenefitthestudyofitsphysiologicalandbiochemicalprocessInthisexperiment，usingthemaizeMDHgeneastheprobe，anovelricefull—lengtheDNAwascloned

4、throughscreeningariceseedlingeDNAlibraryThegene’sfunctionisbrieflystudiedThemainresultspresentedinthisthesisareasfollows1Genecloning：usingthemaizeMDHgeneastheprobe，IscreensariceseedlingcDNAlibrarystrigentlyAnovelricefull

5、—len【gthcDNAwasclonedandwasnamedricecytoplasmicmalicdehydrogenasegene(RcMDH)TheidentityratebetweenRcMDHandthemaizeMDHgeneMcMDHwas87％Itcodesa332aaprotein，andthemolecularweightcloseto365kuThisproteinisthehomologousproteino

6、fmaizeMDH2TheexpressionofthisgeneinEcoli：TheORFofRcMDHwassubclonedintoreconstructedplasmidpGEX4T一1andexpressedinEcolisuccessfullyTheMDHenzymeactivityofitsproductwasalsocertified3TherelationshipbetweenRcMDHandheterosis：Be

7、causeoftheMDHhasveryimportantfunctionsincells，thedifferenceofMDHgene’sexpressionandtheMDHenzymeactivitybetweentheparentsandthehybridF1wascheckedItwascertifiedthattheMDHgene’sexpressionimprovedremarkablyinthehybridF1Keywo