1、青島大學碩士學位論文PIRESegr1IFNγEndostatin聯(lián)合放療抗腫瘤效應的實驗研究姓名：張永春申請學位級別：碩士專業(yè)：腫瘤學指導教師：梁軍20080608AstudyOHtheantitumoreffectsofPIRESegrIIFN7EndostatincombinedwithradiotherapyAbstractObjectiveThemainpurposeofthetestistoinvestigatetheeff

2、ectsofPIRES—egr1一IFN一7一Endostatinlimpsomecombinedirradiationtothetumorinvivo，eventhedepressiveeffectsofendostatinonangiogenesisandimmniolgyeffectsofIFN一丫totumorItwillprovidescientifictheoriesandexperiencebasementstesearc

3、hthegenetherapyinclinicMethodsTothelimipsomeofPIRESegr1一IFN—Y—Endostatin，andinjectthelimipsomesintothetumorsofhepticcarcinomainrats，thengivelocalirradiationtothemassThelimipsomewillbeinducedtoexpressandthegrowthofthemass

4、willbechecked，andthediameterofthemasswillbegettedThentheexpressionlevelsofIFN7andEndostatininmouseserumwithdifferentvectordosageanddifferenttimewasmeasuredbyELISAWecanbiopsythemassandstaininH&EandISHThenumberofvascularin

5、H&EstainingandtheexpressionofCD31inISHstainingwerecountedResultsQThegrowthofthemasswasinhibitedobviouslyinadosedependentandatimedependentmannerafterinjectionofplasmidandradiating，anditwasstatisticallysignificantobviously

6、thattumorgrowthinradiationgroupandplasmidradiationgroupwasslowerthanwhichincontrolgroups(p001)(室)RadiationcaninducetheexpressionofINF7andEndostatininadosedependentmanneranditslevelincreasedmarkedlyandreachedtoapeakatthe3

7、tdweekTheexpressionsofINF吖Canmaintainsteadilyforabout4~5weeks③ThenumberofvascularinH&EstainingandtheexpressionofCD31inISHstainingweredifferentindiferentgroups(P001)ConclusionsRadiationCallinducetheexpressionofIFN7andEndo