1、東南大學碩士學位論文NGF對新生腦神經元細胞缺氧缺血損傷保護作用的研究—NGF對線粒體結構及功能的影響姓名：黃莉申請學位級別：碩士專業(yè)：兒科指導教師：蔣犁20040508ABSTRACTAbstractTitle：TheProtectiveEffectofNGFonHypoxic—ischemicNeuronalCellsiUNeonatalBrain——toExploretheEfkctofNGFonTheirMitochondria

2、lStructureandFunctionPostgraduate：HUANGLiTutor：JIANGLiUniversity：SoutheastUhiversity0bjective：TodeterminetheprotectiveeffectofNGFonhypoxic—ischemic(HI)neuronalcellsofneonatalbraininvitroMethods：(1)HImodelofneuronalcellso

3、fneonatalbraininvitrowasinducedbythedeprivationofoxygenandglucoseaccordingtotheimprovedGoldberg’smethod②Inordertofindouttheoptimalconcentration，MTTmethodwasappliedtomeasuringthevitalityofneuronalcellstreatedwimNGFindiffe

4、rentconcentrationofNGETheresultwascomparedwimthevitalit、rofneuronalcellsinthenormalgroup，andinthecontrol(H11oneatdifierenttimepoints(0、24hoursafterHI)⑨Neuronalcellsinvitroweredividedinto3groups：thenormal、thecontrol(HI)、a

5、ndthetherapeuticgroupThemorphologicchangesofneuronalcellswereobservedbylightandelectronicmicroscopes。TheexpressionValHeofCyt—CandA1FmRNAofdifferentgroupweredeterminedbyimmunocytochemistryandRrrPCRrespectivelyResults：(1)T

6、hevalueofMTTinHIgroupwassignificantlylowerthanthatinthenormalgroupandthebesttherapeauticoneAccordingtothevalueofMTT80ng／mIofNGFiStheoptimalconcentration(④Comparedwiththatofnormalgroupandthetherapeuticone，thevalueofCvtCan

7、dAIFmRNAofthecontr01wassignificantlyhigher伊O05)Therewasriosignificantdifierencebetweenthenorlllalgroupandthetherapeuticone(9“曲tandelectron—microscopicobservationshowedthattheapoptosisandtheabnormalmorphologicchangesofneu

8、ronalcellswereobviousinthetherapeauticgroup@NGFwhichwasusedat240hourafterHIcontrolgroup，comparedwiththoseinthehoursafterH1waslesseffectivethanthatatConclusion：InvitroHImodelofneuronalcellsofneonatalbrainCansimulateHIbrai

9、ndamage(HIBD)ExogenousNGF(80ng／m1)hasthebestprotectiveeffectonHIneuronalcellsTheearlierNGFwasusedthebeRertheresultwasKeywords：Neuronalcells；Hypoxiaischemia；Nervegrowthfactor(NGt)Apoptosis—inducingfactors(AIF)；Cytochromec