1、湖南農業(yè)大學碩士學位論文一種水稻矮化突變體的生理特性及調控研究姓名：史齊申請學位級別：碩士專業(yè)：植物學指導教師：肖浪濤20070601AbstarctTakingrice(OryznSativeL)varietyzhonghuall(ZHll)罄control，theplantmorphological，physiologicalandbiochemicalcharacteristicsandtheregulationofexogeno

2、usgrowthsubstancesofCA648，aricedwarfmutantlineobtainedbytransformationwiththeplasmidpDsl300TDNAintoricevarietyZH1l，werestudiedinthepaperTheplantsofCA648weretreatedwithGA3，IAA，ZandGA3atseedlingsstageandGA3atmidtilleringst

3、age，thenthebiologicalcharacteristicswereanalyzed，andendogenousphytohormones，solublesugar，solubleprotein，theactivitiesofperoxidaseandIAAoxidasewerealsodeterminedn化mainresultsareasfollows：1Thereisnosignificantdifferencesin

4、thesternlengthduringtheseedlingstagebuttherearesignificantdifferencesduringtilleringstagebetweenCA648andZHll111econtentofendogenousGAI4andIAAinCA648islowerattheseedlingstagebuthiigheratmidtilleringstagethanthatinZHI12Aft

5、ertreatedwiththreedifferentplantgrowthregulatorsduringtheseedlingperiod，CA648plantsat2“l(fā)eafstageWassensitivetothethreeplantgrowthregulators，andshowedthemostsensitivitytowardsGA3Thestemlengthandthe2。4sheathelongationrateo

6、fCA648were501％and657％respectivelyandthecorrespondingendogenousphytohormonalcontentswereincreasedaccordingly111eresultsofdifferentdosagesofGA3treatmentatdifferentstagesindicatethatCA648isalireperiodsensitivemutantwithinth

7、ewholelifecycle3Nosignificantdifferencesofsolublesugarandsolubleproteinbutsignificantdifferencesofphotosyntheticindexes，PODactivityandIODactivitywereobservedatthemidtilleringstagebetweenCA648andZHI114daftermidtilleringst

8、age，PODactivitiesofCA648inleafandstemWas430％and168％higherthanthatofZHllTheIODactivitiesofCA648inleavesWas774％hi曲erthanthatofZHll4，TheplantheightandsolubleproteininCA648raisedwiththeconcentrationofGA3ThePOD，IODactivitiesi

9、nlcavesofCA648reducedwiththeconcentrationofGA3，butthePODactivityinthestemofCA648enhancedwiththeconcentrationofGA3TheaboveresultsindicatethatthemorphologicaldifferencesbetweenCA648andZHl1arecorrelatedwithphytohormonessolu