1、浙江大學博士學位論文腫瘤表達譜基因芯片篩選膽囊癌腫瘤相關(guān)基因的研究姓名：馮雪冬申請學位級別：博士專業(yè)：外科學（普通外科學）指導教師：彭淑牖20050401博士季隹靜喜晰江土拿正學院馮量冬ABSTRACTobjective：TofindoutthedifferentialexpressiongenesingallbladdercarcinomabyprofilingcDNAmicroarrayandtOprovidedataforthed

2、iagnosticeDNAmicroarrayThevalidationofthemicroarcaywasconfirmedsimultaneouslyMethods：ThefreshspecimensofgallbladderandnormalcontrolwerepreservedinCryopreservation，thenthetotalRNAwaSextractedanddetectedHybridizationwithBi

3、ostarH40stypeprofilingeDNAmicroarraywasperformedaftertheRNAwaslabeledwithfluorescentprobeTheimagescanmnganddataanalysiswereperformedsubsequentlyFinallytheresultsofhybridizationwerevalidatedbyreal—timePCRResults：61differe

4、ntialexpressiongenesweledefinitedinaLl4specimens，whichwasinvolvedinseveralaspectoftumordevelopmentTherewere17genesdown—ragulatedand44genesupragulatedTherewere9oncogeneandanti—oncogene；7genesaboutapoptosis；6genesaboutSynt

5、hesis，repairandreconstructionofDNA；13genesaboutsignaltransformation；12genesaboutmetabolismandothers8gene，5genesamongthemwereselectedtobeamplificated，andtheexpressionofthemwascoincidentwiththemicroarrayConclusions：Geneexp

6、ressionprofilinginhumangallbladdercarcinomacarlbeanalyzedbycDNAmicroarraysensitively，generously，andeffectivelyTheresultoftheexperimentofmicroarraywasreliableKeywords：GallbladdercarcinomaProfilingeDNAmicroarrayReal—timeRT