1、女RⅢ2日R7Ⅲ毒與大學(xué)碩士學(xué)位論文)淋B管標(biāo)記方法及標(biāo)志物的搽討)胰腺癌淋B管生成∞特征丑其與物學(xué)行為的關(guān)系指導(dǎo)敏師學(xué)科專業(yè)名稱論支援表日期論蔓答辯目期答輯喜女套王席W辯援李玉軍副主任Ⅸ師病理學(xué)與病理生m學(xué)(捕理紀(jì)祥瑞教攫TheInvestigationofLabelledMethodandMarkerofLymphaticEndoutheliumABSERACT0bjectTostudythevalueofdifferentlabe

2、lledmethodsandmakersoflymphaticendouthelium，andtoprovideanidealexperimentmethodininvestigatingtilelymphangiogenesisoftumotMethod5Naseexpressionsandthemmphologyoflymphaticsin30normalgastrointestinaltissueswereobservedwith

3、enzymehistochemistry1mmuno—histochemistrystaining(PicTureTMthreestepsmethod)wasusedtodetectexpressionofVEGFR3andPodoplanininthesame30paraffinembeddedsamplesResults(DLymphaticvesseldensity(LVD)：TheLVDofVEGFR3group(1433_22

4、4)wasobvioslyhigherthanthatof5’Nasegroup(67811161andpodoplaningroup(685084)withsignificantdifferencesbetweenthem“2755，749；P001)Andtherewasnodifferencebetween5’Naseandpodoplanin②Themorphologyoflymphatics：Thelymphaticswith

5、5NaseandpodoplaninpositiveexpressionwerefoundinlaminapropriaandsubmucosaThewallswerethinnerandnoredbloodceHsintheductsThelymphaticswithVEGFR3positiveexpressionwerealsofoundinlaminapropriaandsubmucosaPartofthewaHswerethic

6、ker，andhadredbloodcellsintheducts(鯽llestainingbackground：Comparedwith5’Nasewhichstainingbackgroudwaswheatandcouldn’tclearlyshowthestructuresurroundingthelymphatics，podoplaninandVEGFR一3wereclearandcouldshowthestructurebet

7、terConclusionItwasallidealexperimentmethodtomarkthelymphatics，byexaminingthepodoplaninexpressionwithinmluno—histochemistrystainingPodoplaninwasanavailableantibodyforthespecificstainingoflymphaticsKeyWordsLymphatics；5Nase