1、中國農業(yè)大學博士學位論文雞肉肌苷酸和肌內脂肪含量遺傳規(guī)律及相關候選基因的研究姓名：陳繼蘭申請學位級別：博士專業(yè)：動物遺傳育種與繁殖指導教師：楊寧20040601StudiesonInheritanceandCandidateGenesofInosine一5’MonophosphateandIntramuscularFatContentsinChickenMeatJi1anChenSupervisor：ProfNingYangAbs扛ac

2、tQualityofchickenmeatisbecomingmoreandmoreimportantformeattypechickenbreedingandproduction。Twoimportantquail每attributesIMP(inosine5mono#ospbate)andi粥匿(intramuscularfat)contents，werestudiedinthethesiswithregardtotheireval

3、uation，geneticparameters，andselectionresponseinBeijingYouchickensMeanwhilethepolymorphismsoftwocandidategenesA酗口Di(adenosine5monophosphatedeaminase)andAFABP(adipocytefatryacidbindingproteins)wereevaluated拄spotentialgenet

4、icmarkersfortheimpmvememofmeatquailtyTheresultswere鑫sfollowsResulIsofsensorytestshowedthatIN母playedamajorroleinumalnitasteandditierentIMPcontentsresultedindifierentumamitestowingtointeractionbetweenIMPandglutaminesodiumW

5、iththeincreasingofthestoragetemparatureandtimethemeasuringvaluesofIⅣ驢contentdecreasedIMPcontentsinbreastmuscleweremaximaltwohoursafterslanghteringatroomtemperature，andthevaluedecreasedby16％and55％afterkeeping12hours霍喲00an

6、d24hoursfFO。e1)，respectivel)：IMPcontentkeptat4“Cfor12hoursafterslaughteringwasequaltothatonehourafcerslaughteringkeptatroomtemperatureIMpcontentbegantodecreaseamonthlaterwhilekeptat15℃fP《001)Whilecomparingmeatumamitastei

7、twasbettertorisecorrectedvaluesof姻contentbasedonIMPrelativesThevalueofIMPcontentvariedwhendisposalliquidwasfrozenforIMPcontentThe腳contentsofBeijingYouchickenwere106—130timesofArborAcars(AA)broilersandthoseofthebreastmusc

8、leware113148timesof1egmusclerespectivelyThemalesandfemalesappearedasimilartrendinIMPcontentIMFcontentofBeijingYouchickenwassignificantlydifferentfromth敲ofAAbroilerlMFcontentofbreastmeatwasonly22％36％ofthatoflegmuscle。玲正co

9、ntent，unrelatedtosex，variedwithweightgainandmcreasedwithageindaysGeneticparametersofBeijingYouehickenworeestimatedTheresultsshowedthatheritabilitiesofIMPand㈣contentswere0，23and010respectivetyThegeneticcorrelationbetweenI

10、MPandIMFcontentswaspositiveandsimilarrelationbetween1MFcontentandbedyweightwas曲served。IMPcontenthoweverwasnegativelyrelatedwithbodyweight，TheresultsofselectiontrialforIMPandIMF搿ivergentlydelected)sbowedthattherealizedhar

11、itabilitiesofIMPandIMFcontentswerehighefthaneatimatedonesandtherealizedgeneticcorrelationofthemweresimilartoestimatedonesTheselectionexperimentdemonstratedthattheI～辨andIMFcontentscouldbeinereasedthroughdirectionalselecti

12、onTbeiocusPBofcandidategeneA腳jshowedpolymorphlsmmdifferentchickenbreeds，butthedistributionofallelefrequencywasnotrelatedtomeatflavortraits，Di自ferentgenotypesofPBloctlsshowedsignificanteriectsonI～Ⅲcontentbuttheeriectsvari

13、edindifferentpopulationsMoreworkareneededtoverifythegeneticeffectsof也egene。T虹chickenswithgenotypeBBoflocusP4AFABPgenehadhigher阱contentthanthosewithothertwogeno夸pes㈨madAB)，GeneticcorrelationsbetweonIMFcontentandabdominalf